Screening and characterization of cellulolytic molds from empty fruit bunches and soils in palm oil plantation area in Indonesia

Miller T., Waturangi D.E., Purwadaria T.

Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman 51, Jakarta, 12930, Indonesia


Abstract

Objective: This research was aimed to isolate cellulolytic molds in empty fruit bunches of oil palm (EFBOP) and soils from palm oil plantation area and identify their enzyme activities to digest EFBOP. Results: A total of seven molds were successfully isolated and screened for their enzyme activities from EFBOP and the soils. The enzymes from each isolate were produced in submerged culture using Mineral Mandels and 3% of alkali pretreated pollard in triplicates. The results indicated that all of the isolates were able to hydrolyze Carboxymethyl Cellulose (CMC), Whatmann No. 1 filter paper, and also EFBOP to sugars with reducing ends that reacted to 3,5-Dinitrosalicylic acid (DNS). The CMCase activity of isolate X showed the highest while the lowest was found for isolate MT8. Filter paperase (FPase) activity of isolate X performed the highest wile the lowest were found from isolate MT3 and MT6. The saccharification activity of isolate P showed the highest while MT6 performed the lowest activity © 2021, The Author(s).

Cellulolytic molds; Empty fruit bunches of oil palm; Enzyme activity


Journal

BMC Research Notes

Publisher: BioMed Central Ltd

Volume 14, Issue 1, Art No 249, Page – , Page Count


Journal Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109019138&doi=10.1186%2fs13104-021-05668-8&partnerID=40&md5=0b1ade93701b3bb71d7f24b89969c257

doi: 10.1186/s13104-021-05668-8

Issn: 17560500

Type: All Open Access, Gold, Green


References

Hoe, T.K., Sarmidi, M.R., Alwee, S.S.R.S., Zakaria, Z.A., Recycling of oil palm empty fruit bunch as potential carrier for biofertilizer formulation (2016) J Technol, 78 (2), pp. 165-170; Li, Q., Ng, W.T., Wu, J.C., Isolation, characterization and application of a cellulose-degrading strain Neurospora crassa S1 from oil palm empty fruit bunch (2014) Microb Cell Fact, 13, pp. 1-8; Sapareng, S., Ala, A., Kuswinanti, T., Rasyid, B., Isolation and characteristics molding fungus of stalks and empty fruit bunches of oil palms (2018) Int J Sci Technol Res, 7 (11), pp. 135-140; Sari, A.R., Kusdiyantini, E., Rukmi, M.G.I., Cellulase production by Aspergillus sp. the result of isolation from sago processing waste (Metroxylon sp.) with variations in the concentration of inoculum in static submerged fermentation (2017) J Biol, 6, pp. 11-20; Talantan, V.M., Marina, L.O., Suwastika, I.N., Cellulase activity from cellulolytic fungi from the soil of Lake Kalimpa’a, Central Sulawesi (2018) J Sci Technol, 7, pp. 323-333; Saxena, J., Pant, V., Sharma, M.M., Gupta, S., Singh, A., Hunt for cellulase producing fungi from soil samples (2015) J Pure Appl Microbiol, 9, pp. 2895-2902. , COI: 1:CAS:528:DC%2BC2sXlsFynsw%3D%3D; Purwadaria, T., Marbun, P.A., Sinurat, A.P., Ketaren, P.P., Comparison of cellulase enzyme activity from bacteria and molds isolated from termites (2003) JITV, 8, pp. 213-219; Muryanto, S.Y., Abimanyu, H., Optimization of the oil palm empty bunches NaOH pretreatment process to become bioethanol (2016) Indones J Appl Chem, 18, pp. 27-35; Purwadaria, T., Kumalasari, A.T., Tutiharyati, K.P.P., Sinurat, A.P., Optimization of cellulase production with Penicillium nalgiovense S11 grown on pretreated wheat pollard (2004) Biotropia, 23, pp. 1-12; Miller, G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar (1959) Anal Chem, 31, pp. 426-428. , COI: 1:CAS:528:DyaG1MXmtFKiuw%3D%3D; Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochcm, 72, pp. 248-254. , COI: 1:CAS:528:DyaE28XksVehtrY%3D; Purwadaria, T., Ketaren, P.P., Sinurat, A.P., Sutikno, I., Identification and evaluation of fiber hydrolytic enzymes in the extract of termites (Glyptotermes montanus) for poultry feed application (2003) Indones J Agric Sci, 4 (2), pp. 40-47; Mandal, M., Ghosh, U., Application of statistical tool for optimization of physical parameters for cellulase production under solid state fermentation (2018) Indian J Biotechnol, 17, pp. 441-447. , COI: 1:CAS:528:DC%2BC1MXhtlemsrjJ; Karmawan, L.U., Dwivany, F.M., Esyanti, R.R., Aryantha, I.N.P., Pre-treatment and suitable reagent enabled a reliable and consistent for molecular detection of Fusarium oxysporum fsp cubense tropical race 4 (Foc TR4) (2019) HAYATI J Biosci., 26 (4), pp. 196-203; Sudiyani, Y., Styarini, D., Triwahyuni, E., Sudiyarmanto, S.K.C., Aristiawan, Y., Abimanyu, H., Han, M.H., Utilization of biomass waste empty fruit bunch fiber of palm oil for bioethanol production using pilot–scale unit (2013) Energy Proc, 32, pp. 31-38

Indexed by Scopus

Leave a Comment