Imron M.F., Kurniawan S.B., Abdullah S.R.S.
Department of Biology, Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, Bangi, 43600, Malaysia
Leachate is produced from sanitary landfills containing various pollutants, including heavy metals. This study aimed to determine the resistance of bacteria isolated from non-active sanitary landfill leachate to various heavy metals and the effect of salinity levels on the removal of Hg by the isolated bacterium. Four dominant bacteria from approximately 33 × 1017 colony-forming units per mL identified as Vibrio damsela, Pseudomonas aeruginosa, Pseudomonas stutzeri, and Pseudomonas fluorescens were isolated from non-active sanitary landfill leachate. Heavy metal resistance test was conducted for Hg, Cd, Pb, Mg, Zn, Fe, Mn, and Cu (0–20 mg L− 1). The removal of the most toxic heavy metals by the most resistant bacteria was also determined at different salinity levels, i.e., fresh water (0‰), marginal water (10‰), brackish water (20‰), and saline water (30‰). Results showed that the growth of these bacteria is promoted by Fe, Mn, and Cu, but inhibited by Hg, Cd, Pb, Mg, and Zn. The minimum inhibitory concentration (MIC) of all the bacteria in Fe, Mn, and Cu was > 20 mg L− 1. The MIC of V. damsela was 5 mg L− 1 for Hg and > 20 mg L− 1 for Cd, Pb, Mg, and Zn. For P. aeruginosa, MIC was > 20 mg L− 1 for Cd, Pb, Mg, and Zn and 10 mg L− 1 for Hg. Meanwhile, the MIC of P. stutzeri was > 20 mg L− 1 for Pb, Mg, and Zn and 5 mg L− 1 for Hg and Cd. The MIC of P. fluorescens for Hg, Pb, Mg, and Zn was 5, 5, 15, and 20 mg L− 1, respectively, and that for Cd was > 20 mg L− 1. From the MIC results, Hg is the most toxic heavy metal. In marginal water (10‰), P. aeruginosa FZ-2 removed up to 99.7% Hg compared with that in fresh water (0‰), where it removed only 54% for 72 h. Hence, P. aeruginosa FZ-2 is the most resistant to heavy metals, and saline condition exerts a positive effect on bacteria in removing Hg. © 2021, The Author(s).
Bioremediation; Heavy metal resistance; Leachate; Mercury; Minimum inhibitory concentration; Pseudomonas aeruginosa
Sustainable Environment Research
Publisher: BioMed Central Ltd
Volume 31, Issue 1, Art No 14, Page – , Page Count
Journal Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102977383&doi=10.1186%2fs42834-021-00088-6&partnerID=40&md5=92d4d22844f0f0a5941804dfe6d0310e
Type: All Open Access, Gold
Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B.B., Beeregowda, K.N., Toxicity, mechanism and health effects of some heavy metals (2014) Interdiscip Toxicol., 7, pp. 60-72; Bartkowiak, A., Breza-Boruta, B., Lemanowicz, J., Assessment of the content of heavy metals and potential pathogenic microorganisms in soil under illegal dumping sites (2016) Environ Earth Sci., 75, p. 1401; Vodyanitskii, Y.N., Biochemical processes in soil and groundwater contaminated by leachates from municipal landfills (mini review) (2016) Ann Agrar Sci., 14, pp. 249-256; Teta, C., Hikwa, T., Heavy metal contamination of ground water from an unlined landfill in Bulawayo, Zimbabwe (2017) J Health Pollut., 7, pp. 18-27; Agamuthu, P., Fauziah, S.H., (2010) Heavy metal pollution in landfill environment: a Malaysian case study, , 4th International Conference on Bioinformatics and Biomedical Engineering, Chengdu: Jun 18–20; Xue, Q., Li, J.S., Wang, P., Liu, L., Li, Z.Z., Removal of heavy metals from landfill leachate using municipal solid waste incineration fly ash as adsorbent (2014) Clean-Soil Air Water., 42, pp. 1626-1631; Thompson, L.A., Darwish, W.S., Environmental chemical contaminants in food: review of a global problem (2019) J Toxicol-US., 2019, p. 2345283; Gworek, B., Dmuchowski, W., Gozdowski, D., Koda, E., Osiecka, R., Borzyszkowski, J., Influence of a municipal waste landfill on the spatial distribution of mercury in the environment (2015) PLoS One., 10; Maiti, S.K., De, S., Hazra, T., Debsarkar, A., Dutta, A., Characterization of leachate and its impact on surface and groundwater quality of a closed dumpsite – a case study at Dhapa, Kolkata, India (2016) Procedia Environ Sci., 35, pp. 391-399; Grassi, S., Netti, R., Sea water intrusion and mercury pollution of some coastal aquifers in the province of Grosseto (Southern Tuscany – Italy) (2000) J Hydrol., 237, pp. 198-211; Umar, M., Aziz, H.A., Yusoff, M.S., Variability of parameters involved in leachate pollution index and determination of LPI from four landfills in Malaysia (2010) Int J Chem Eng., 2010, p. 747953; Mangimbulude, J.C., Goeltom, M.T., van Breukelen, B.M., van Straalen, N.M., Roling, W.F.M., Hydrochemical characterization of a tropical, coastal aquifer affected by landfill leachate and seawater intrusion (2016) Asian J Water Environ., 13, pp. 49-57; Greene, R., Timms, W., Rengasamy, P., Arshad, M., Cresswell, R., Soil and aquifer salinization: toward an integrated approach for salinity management of groundwater (2016) Integrated groundwater management, pp. 377-412. , Jakeman AJ, Barreteau O, Hunt RJ, Rinaudo JD, Ross A, (eds), Springer, Cham; Awasthi, A.K., Pandey, A.K., Khan, J., Biosorption an innovative tool for bioremediation of metal-contaminated municipal solid waste leachate: optimization and mechanisms exploration (2017) Int J Environ Sci Te., 14, pp. 729-742; Imron, M.F., Kurniawan, S.B., Soegianto, A., Characterization of mercury-reducing potential bacteria isolated from Keputih non-active sanitary landfill leachate, Surabaya, Indonesia under different saline conditions (2019) J Environ Manage., 241, pp. 113-122; Latorre, I., Hwang, S., Montalvo-Rodriguez, R., Isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating PVC materials (2012) J Environ Sci Heal A., 47, pp. 2254-2262; Arab, B., Hassanpour, F., Arshadi, M., Yaghmaei, S., Hamedi, J., Optimized bioleaching of copper by indigenous cyanogenic bacteria isolated from the landfill of e-waste (2020) J Environ Manage., 261, p. 110124; Imron, M.F., Kurniawan, S.B., Soegianto, A., Identification of mercury-resistant bacteria as a potential candidate for mercury bioremediation (2019) Ecol Environ Conserv., 25, pp. S66-S69; Sanuth, H.A., Adekanmbi, A.O., Biosorption of heavy metals in dumpsite leachate by metal-resistant bacteria isolated from Abule-egba dumpsite, Lagos State, Nigeria (2016) Brit Microbiol Res J., 17, pp. 1-8; Neneng, L., Gunawan, Y.E., The role of coenzymes on mercury (Hg2+) bioremediation by isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 (2018) J Trop Life Sci., 8, pp. 16-20; De, J., Leonhauser, J., Vardanyan, L., Removal of mercury in fixed-bed continuous upflow reactors by mercury-resistant bacteria and effect of sodium chloride on their performance (2014) QScience Connect., 2014, p. 17; Dash, H.R., Mangwani, N., Das, S., Characterization and potential application in mercury bioremediation of highly mercury-resistant marine bacterium Bacillus thuringiensis PW-05 (2014) Environ Sci Pollut R., 21, pp. 2642-2653; Imron, M.F., Kurniawan, S.B., Titah, H.S., Potential of bacteria isolated from diesel-contaminated seawater in diesel biodegradation (2019) Environ Technol Inno., 14, p. 100368; (2012) Standard methods for examination of water and wastewater, , 22, American Public Health Association, Washington, DC; (1978) Phosphorous, all forms (colorimetric, ascorbic acid, two reagent), , US Environmental Protection Agency, Washington, DC; Giovanella, P., Cabral, L., Costa, A.P., Camargo, F.A.D., Gianello, C., Bento, F.M., Metal resistance mechanisms in Gram-negative bacteria and their potential to remove Hg in the presence of other metals (2017) Ecotox Environ Safe., 140, pp. 162-169; Kurniawan, S.B., Imron, M.F., Seasonal variation of plastic debris accumulation in the estuary of Wonorejo River, Surabaya, Indonesia (2019) Environ Technol Inno., 16, p. 100490; Kurniawan, S.B., Imron, M.F., The effect of tidal fluctuation on the accumulation of plastic debris in the Wonorejo River Estuary, Surabaya, Indonesia (2019) Environ Technol Inno, 15, p. 100420; Murinova, S., Dercova, K., Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane (2014) Int J Microbiol., 2014, p. 873081; Igiri, B.E., Okoduwa, S.I.R., Idoko, G.O., Akabuogu, E.P., Adeyi, A.O., Ejiogu, I.K., Toxicity and bioremediation of heavy metals contaminated ecosystem from tannery wastewater: a review (2018) J Toxicol-US., 2018, p. 2568038; Ianeva, O.D., Mechanisms of bacteria resistance to heavy metals (2009) Mikrobiol Z., 71, pp. 54-65. , [in Russian]; Banerjee, A., Das, D., Rudra, S.G., Mazumder, K., Andler, R., Bandopadhyay, R., Characterization of exopolysaccharide produced by Pseudomonas sp. PFAB4 for synthesis of EPS-coated AgNPs with antimicrobial properties (2020) J Polym Environ., 28, pp. 242-256; Zheng, R.K., Wu, S.M., Ma, N., Sun, C.M., Genetic and physiological adaptations of marine bacterium Pseudomonas stutzeri 273 to mercury stress (2018) Front Microbiol., 9, p. 682; Chellaiah, E.R., Cadmium (heavy metals) bioremediation by Pseudomonas aeruginosa: a minireview (2018) Appl Water Sci., 8, p. 154; Naik, M.M., Dubey, S.K., Khanolkar, D., D’Costa, B., P-type ATPase and MdrL efflux pump-mediated lead and multi-drug resistance in estuarine bacterial isolates (2013) Curr Sci India., 105, pp. 1366-1372; Miller, C.D., Pettee, B., Zhang, C., Pabst, M., McLean, J.E., Anderson, A.J., Copper and cadmium: responses in Pseudomonas putida KT2440 (2009) Lett Appl Microbiol., 49, pp. 775-783; Jaroslawiecka, A., Piotrowska-Seget, Z., Lead resistance in micro-organisms (2014) Microbiol-SGM., 160, pp. 12-25; Al-Aoukaty, A., Appanna, V.D., Huang, J., Exocellular and intracellular accumulation of lead in Pseudomonas fluorescens ATCC 13525 is mediated by the phosphate content of the growth medium (1991) FEMS Microbiol Lett., 83, pp. 283-290; Prabhakaran, P., Ashraf, M.A., Aqma, W.S., Microbial stress response to heavy metals in the environment (2016) RSC Adv., 6, pp. 109862-109877; Khatiwada, B., Hasan, M.T., Sun, A., Kamath, K.S., Mirzaei, M., Sunna, A., Probing the role of the chloroplasts in heavy metal tolerance and accumulation in Euglena gracilis (2020) Microorganisms., 8, p. 115; Marsidi, N., Hasan, H.A., Halmi, M.I.E., Abdullah, S.R.S., Resistance of native bacteria isolated from activated sludge towards iron and manganese (2019) Malaysian J Anal Sci., 23, pp. 131-137; Purwanti, I.F., Kurniawan, S.B., Imron, M.F., Potential of Pseudomonas aeruginosa isolated from aluminium-contaminated site in aluminium removal and recovery from wastewater (2019) Environ Technol Inno., 15, p. 100422; Saranya, K., Sundaramanickam, A., Shekhar, S., Swaminathan, S., Balasubramanian, T., Bioremediation of mercury by Vibrio fluvialis screened from industrial effluents (2017) Biomed Res Int., 2017, p. 6509648; Giovanella, P., Cabral, L., Bento, F.M., Gianello, C., Camargo, F.A.O., Mercury (II) removal by resistant bacterial isolates and mercuric (II) reductase activity in a new strain of Pseudomonas sp. B50A (2016) New Biotechnol., 33, pp. 216-223; Kotwal, D.R., Shewale, N.B., Tambat, U.S., Thakare, M.J., Bholay, A.D., Bioremediation of mercury using mercury resistant bacteria (2018) Res J Life Sci Bioinf Pharm Chem Sci., 4, pp. 145-156; Pepi, M., Gaggi, C., Bernardini, E., Focardi, S., Lobianco, A., Ruta, M., Mercury-resistant bacterial strains Pseudomonas and Psychrobacter spp. isolated from sediments of Orbetello Lagoon (Italy) and their possible use in bioremediation processes (2011) Int Biodeter Biodegr., 65, pp. 85-91; Yan, N., Marschner, P., Cao, W.H., Zuo, C.Q., Qin, W., Influence of salinity and water content on soil microorganisms (2015) Int Soil Water Conse., 3, pp. 316-323; Deng, X., Wang, P.T., Isolation of marine bacteria highly resistant to mercury and their bioaccumulation process (2012) Bioresour Technol., 121, pp. 342-347; Al-Qadiri, H.M., Al-Holy, M.A., Lin, M.S., Alami, N.I., Cavinato, A.G., Rasco, B.A., Rapid detection and identification of Pseudomonas aeruginosa and Escherichia coli as pure and mixed cultures in bottled drinking water using Fourier transform infrared spectroscopy and multivariate analysis (2006) J Agr Food Chem., 54, pp. 5749-5754; Purwanti, I.F., Kurniawan, S.B., Ismail, N.I., Imron, M.F., Abdullah, S.R.S., Aluminium removal and recovery from wastewater and soil using isolated indigenous bacteria (2019) J Environ Manage., 249, p. 109412; Hasan, H.A., Abdullah, S.R.S., Kofli, N.T., Yeoh, S.J., Interaction of environmental factors on simultaneous biosorption of lead and manganese ions by locally isolated Bacillus cereus (2016) J Ind Eng Chem., 37, pp. 295-305; Elham, O.S.J., Muda, S.A., Abu Hasan, H., Abdullah, S.R.S., Biological treatment of Pb and Zn using sequencing batch reactor (2018) J Kejuruter., 30, pp. 201-207; Subari, F., Kamaruzzaman, M.A., Abdullah, S.R.S., Abu Hasan, H., Othman, A.R., Simultaneous removal of ammonium and manganese in slow sand biofilter (SSB) by naturally grown bacteria from lake water and its diverse microbial community (2018) J Environ Chem Eng., 6, pp. 6351-6358; Sinha, A., Pant, K.K., Khare, S.K., Studies on mercury bioremediation by alginate immobilized mercury tolerant Bacillus cereus cells (2012) Int Biodeter Biodegr., 71, pp. 1-8; Purwanti, I.F., Obenu, A., Tangahu, B.V., Kurniawan, S.B., Imron, M.F., Abdullah, S.R.S., Bioaugmentation of Vibrio alginolyticus in phytoremediation of aluminium-contaminated soil using Scirpus grossus and Thypa angustifolia (2020) Heliyon., 6; Kamaruzzaman, M.A., Abdullah, S.R.S., Hasan, H.A., Hassan, M., Othman, A.R., Idris, M., Characterisation of Pb-resistant plant growth-promoting rhizobacteria (PGPR) from Scirpus grossus (2020) Biocatal Agric Biote., 23, p. 101456; Jayanthi, B., Emenike, C.U., Auta, S.H., Agamuthu, P., Fauziah, S.H., Characterization of induced metal responses of bacteria isolates from active non-sanitary landfill in Malaysia (2017) Int Biodeter Biodegr., 119, pp. 467-475
Indexed by Scopus