Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against betaamyloid neurotoxicity on primary culture of nerve cells induced by 2-methoxyethanol [Potencial neuroprotetor do agente trans-resveratrol em cascas de sementes de soja e tempê derivadas da neurotoxicidade beta-amiloide na cultura primária de células nervosas induzidas pelo 2-metoxietanol]

Irnidayanti Y., Sutiono D.R., Ibrahim N., Wisnuwardhani P.H., Santoso A.

Universitas Negeri Jakarta, Faculty of Mathematics and Science, Department of Animal Development Biology, Jakarta, Indonesia; Jakarta State University, Faculty of Mathematics and Natural Sciences, Research Group of Biology, Jakarta, Indonesia; Universitas Indonesia, Faculty of Medicine, Jakarta, Indonesia; Indonesian Institute of Sciences-LIPI, Biotechnology, Bogor, Indonesia


Abstract

Resveratrol, a natural polyphenol found in tempeh, has not been investigated especially in vitro as a neuroprotective agent against 2-methoxyethanol (2-ME)-induced beta-amyloid cytotoxicity. Beta amyloid peptides (Aβ) could initiate neurotoxic events and neuron-inflammatory response via microglial activation. However, it remains unknown whether the neurotoxic effect of beta-amyloid and/or associated with the potential of 2-ME to induce neurotoxic effects on primary culture of nerve cells induced by 2-ME. This study investigated potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid cytotoxicity on primary culture of nerve cells induced by 2-methoxyethanol. Biotium and MTT assays were used to analyze neurons, which were isolated from the cerebral cortex of fetal mice at gestation day 19 (GD-19). A standard solution of 2-methoxyethanol was dosed at 10 μL. The cultured cells were randomly divided into the following groups: (1) 2-ME group+resveratrol standard, (2) 2-ME group+resveratrol isolated from tempeh, (3) 2-ME group+resveratrol isolated from soybean seed coats, and (4) the control group, without the addition of either 2-ME or resveratrol. Exposure of the primary cortical neuron cells to beta-amyloid monoclonal antibody pre-incubated for 24 h with 10 µL of 4.2 µg/mL resveratrol and 7.5 mmol/l 2-methoxy-ethanol additions. Here, we report that the addition of 2-ME and resveratrol (standard and isolated from tempeh) of cell culture at concentrations of 1.4, 2.8 and 4.2 µg/mL showed that the majority of neurons grew well. In contrast, after exposure to 2-ME and Beta-amyloid, showed that glial activated. These findings demonstrate a role for resveratrol in neuroprotective-neurorescuing action. © 2022, Instituto Internacional de Ecologia. All rights reserved.

2-methoxyethanol; Neuroprotective; Neurorescuing; Neurotoxic; Resveratrol


Journal

Brazilian Journal of Biology

Publisher: Instituto Internacional de Ecologia

Volume 82, Issue , Art No e235781, Page – , Page Count


Journal Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103600841&doi=10.1590%2f1519-6984.235781&partnerID=40&md5=f9c209be3a962349c4dffbe941a9af26

doi: 10.1590/1519-6984.235781

Issn: 15196984

Type: All Open Access, Gold


References

ADIBI, J.J., WHYATT, R.M., WILLIAMS, P.L., CALAFAT, A.M., CAMANN, D., HERRICK, R., NELSON, H., HAUSER, R., Characterization of phthalate exposure among pregnant women assessed by repeat air and urine samples (2008) Environmental Health Perspectives, 116 (4), pp. 467-473. , http://dx.doi.org/10.1289/ehp.10749, PMid:18414628; ALMEIDA, L., VAZ-DA-SILVA, M., FALCÃO, A., SOARES, E., COSTA, R., LOUREIRO, A.I., FERNANDES-LOPES, C., SOARES-DA-SILVA, P., Pharmacokinetic and safety profile of trans-resveratrol in a rising multiple dose study in healthy volunteers (2009) Molecular Nutrition & Food Research, 53, pp. s7-s15. , http://dx.doi.org/10.1002/mnfr.200800177, suppl. 1, PMid:19194969; BAUR, J.A., SINCLAIR, D.A., Therapeutic potential of resveratrol : The in vivo evidence (2006) Nature Reviews. Drug Discovery, 5 (6), pp. 493-506. , http://dx.doi.org/10.1038/nrd2060, PMid:16732220; BEHRENS, M.M., STRASSER, U., KOH, J.Y., GWAG, B.J., CHOI, D.W., Prevention of neuronal apoptosis by phorbol ester-induced activation of protein kinase C: Blockade of p38 mitogen-activated protein kinase (1999) Neuroscience, 94 (3), pp. 917-927. , http://dx.doi.org/10.1016/S0306-4522(99)00212-2, PMid:10579584; BELLAVER, B., SOUZA, D.G., BOBERMIN, L.D., SOUZA, D.O., GONCALVES, C.A., QUINCOZES-SANTOS, A., (2015); Resveratrol protects hippocampal astrocytes against LPS-induced neurotoxicity through HO-1, p38 and ERK pathways Neurochemical Research, 40 (8), pp. 1600-1608. , http://dx.doi.org/10.1007/s11064-015-1636-8, PMid:26088684; CORDEY, M., GUNDIMEDA, U., GOPALAKRISHNA, R., PIKE, C.J., Estrogen activates protein kinase C in neurons: Role in neuroprotection (2003) Journal of Neurochemistry, 84 (6), pp. 1340-1348. , http://dx.doi.org/10.1046/j.1471-4159.2003.01631.x, PMid:12614334; DAVEREY, A., AGRAWAL, S.K., Pre and post treatment with curcumin and resveratrol protects astrocytes after oxidative stress (2018) Brain Research, 1692, pp. 45-55. , http://dx.doi.org/10.1016/j.brainres.2018.05.001, PMid:29729252; DORÉ, S., TAKAHASHI, M., FERRIS, C.D., ZAKHARY, R., HESTER, L.D., GUASTELLA, D., SNYDER, S.H., Bilirubin, formed by activation of heme oxygenase-2, protects neurons against oxidative stress injury (1999) Proceedings of the National Academy of Sciences of the United States of America, 96 (5), pp. 2445-2450. , http://dx.doi.org/10.1073/pnas.96.5.2445, PMid:10051662; GESSLER, M.C., NKUNYA, M.H., MWASUMBI, L.B., HEINRICH, M., TANNER, M., Screening tanzanian medical plants for antimalarial activity (1994) Acta Tropica, 56 (1), pp. 65-77. , http://dx.doi.org/10.1016/0001-706X(94)90041-8, PMid:8203297; GOMES, B.A.Q., SILVA, J.P.B., ROMEIRO, C.F.R., SANTOS, S.M., RODRIGUES, C.A., GONÇALVES, P.R., SAKAI, J.T., MONTEIRO, M.C., Neuroprotective mechanisms of resveratrol in Alzheimer’s disease: Role of SIRT1 (2018) Oxidative Medicine and Cellular Longevity, 2018, p. 8152373. , http://dx.doi.org/10.1155/2018/8152373, PMid:30510627; HAN, Y.S., ZHENG, W.H., BASTIANETTO, S., CHABOT, J.G., QUIRION, R., Neuroprotective effects of resveratrol against β-amyloid-induced neurotoxicity in rat hippocampal neurons: Involvement of protein kinase C (2004) British Journal of Pharmacology, 141 (6), pp. 997-1005. , http://dx.doi.org/10.1038/sj.bjp.0705688, PMid:15028639; HARDY, J., SELKOE, D.J., The amyloid hypothesis of Alzheimer’s disease: Progress and problems on the road to therapeutics (2002) Science, 297 (5580), pp. 353-356. , http://dx.doi.org/10.1126/science.1072994, PMid:12130773; IRNIDAYANTI, Y., SUTIONO, D.R., Tempeh & Soybean seed coat: The alternative sources of trans-resveratrol as neuroprotective agents (2019) International Journal of Morphology, 37 (3), pp. 1164-1171. , http://dx.doi.org/10.4067/S0717-95022019000301164; IRNIDAYANTI, Y., SUTIONO, D.R., Unlighted maceration and ultrasound technique: The key to gaining stable transresveratrol from alternative sources in tempeh and soybean seed coat (2019) Pakistan Journal of Nutrition, 18 (6), pp. 548-553. , http://dx.doi.org/10.3923/pjn.2019.548.553; IRNIDAYANTI, Y., DARMANTO, W., ABADI, A., HATTORI, Y., YAMASHIRO, Y., Differential expression of Vimentin and GFAP protein during brain development of mouse fetuses after treated with 2-methoxyethanol (2013) ITB Journal of Science, 44 (4), pp. 346-357. , http://dx.doi.org/10.5614/itbj.sci.2012.44.4.5; JANG, J.H., SURH, Y.J., Protective effects of resveratrol on b-amyloid-induced oxidative PC12 cell death (2003) Free Radical Biology & Medicine, 34 (8), pp. 1100-1110. , http://dx.doi.org/10.1016/S0891-5849(03)00062-5, PMid:12684095; JOHANSON, G., Toxicity review of ethylene glycol monomethyl ether and its acetate ester (2000) Critical Reviews in Toxicology, 30 (3), pp. 307-345. , http://dx.doi.org/10.1080/10408440091159220, PMid:10852499; KAPCZINSKI, F., FREY, B.N., KAUER-SANT’ANNA, M., GRASSI-OLIVEIRA, R., Brain-derived neurotrophic factor and neuroplasticity in bipolar disorder (2008) Expert Review of Neurotherapeutics, 8 (7), pp. 1101-1113. , http://dx.doi.org/10.1586/14737175.8.7.1101, PMid:18590480; KIRK, R.E., OTHMER, D.F., (1980) Encyclopedia of chemical technology, , 3rd ed. New York: John Wiley & Sons; LANGE, W., LI, S., Resveratrol, pterostilbene, and dementia (2018) BioFactors, 44 (1), pp. 83-90. , http://dx.doi.org/10.1002/biof.1396, PMid:29168580; LU, K.T., CHIOU, R.Y., CHEN, L.G., CHEN, M.H., TSENG, W.T., HSIEH, H.T., YANG, Y.L., Neuroprotective effects of resveratrolon cerebral ischemia-induced neuron loss mediated by free radical scavenging and cerebral blood flow elevation (2006) Journal of Agricultural and Food Chemistry, 54 (8), pp. 3126-3131. , http://dx.doi.org/10.1186/1742-2094-7-46, http://dx.doi.org/10.1021/jf053011q, PMid:16608241. LU, X., MA, L., RUAN, L., KONG, Y., MOU, H., ZHANG, Z., WANG, Z., WANG, J.M. and LE, Y., 2010. Resveratrol differentially modulates inflammatory responses of microglia and astrocytes. Journal of Neuroinflammation, 7 1, 46. PMid:20712904; MAHER, P., How protein kinase C activation protects nerve cells from oxidative stress-induced cell death (2001) Journal of Neuroscience Research, 21 (9), pp. 2929-2938. , PMid:11312276; MATSUO, M., SASAKI, N., SAGA, K., KANEKO, T., Cytotoxicity of flavonoids toward cultured normal human cells (2005) Biological & Pharmaceutical Bulletin, 28 (2), pp. 253-259. , http://dx.doi.org/10.1248/bpb.28.253, PMid:15684479; MOLOFSKY, A.V., KRENICK, R., ULLIAN, E., TSAI, H., DENEEN, B., RICHARDSON, W.D., BARRES, B.A., ROWITCH, D.H., Astrocytes and disease: A neurodevelopmental perspective (2012) GENE & DEV, 26 (9), pp. 891-907. , http://dx.doi.org/10.1101/gad.188326.112, PMid:22549954; OKADA, S., HARA, M., KOBAYAKAWA, K., MATSUMOTO, Y., NAKASHIMA, Y., Astrocyte reactivity and astrogliosis after spinal cord injury (2018) Journal of Neuroscience Research, 126, pp. 39-43. , http://dx.doi.org/10.1016/j.neures.2017.10.004, PMid:29054466; PHATNANI, H., MANIATIS, T., Astrocytes in neurodegenerative disease (2015) Cold Spring Harbor Perspectives in Biology, 7 (6), p. a020628. , http://dx.doi.org/10.1101/cshperspect.a020628, PMid:25877220; SUN, A.Y., WANG, Q., SIMONYI, A., SUN, G.Y., Resveratrol as a therapeutic agent for neurodegenerative diseases (2010) Molecular Neurobiology, 41 (2-3), pp. 375-383. , http://dx.doi.org/10.1007/s12035-010-8111-y, PMid:20306310; TANG, F., GUO, S., LIAO, H., YU, P., WANG, L., SONG, X., CHEN, J., YANG, Q., Resveratrol enhances neurite outgrowth and synaptogenesis via sonic hedgehog signaling following oxygen-glucose deprivation/reoxygenation injury (2017) Cellular Physiology and Biochemistry, 43 (2), pp. 852-869. , http://dx.doi.org/10.1159/000481611, PMid:28957797

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