Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against betaamyloid neurotoxicity on primary culture of nerve cells induced by 2-methoxyethanol [Potencial neuroprotetor do agente trans-resveratrol em cascas de sementes de soja e tempê derivadas da neurotoxicidade beta-amiloide na cultura primária de células nervosas induzidas pelo 2-metoxietanol]

Irnidayanti Y., Sutiono D.R., Ibrahim N., Wisnuwardhani P.H., Santoso A.

Universitas Negeri Jakarta, Faculty of Mathematics and Science, Department of Animal Development Biology, Jakarta, Indonesia; Jakarta State University, Faculty of Mathematics and Natural Sciences, Research Group of Biology, Jakarta, Indonesia; Universitas Indonesia, Faculty of Medicine, Jakarta, Indonesia; Indonesian Institute of Sciences-LIPI, Biotechnology, Bogor, Indonesia


Resveratrol, a natural polyphenol found in tempeh, has not been investigated especially in vitro as a neuroprotective agent against 2-methoxyethanol (2-ME)-induced beta-amyloid cytotoxicity. Beta amyloid peptides (Aβ) could initiate neurotoxic events and neuron-inflammatory response via microglial activation. However, it remains unknown whether the neurotoxic effect of beta-amyloid and/or associated with the potential of 2-ME to induce neurotoxic effects on primary culture of nerve cells induced by 2-ME. This study investigated potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid cytotoxicity on primary culture of nerve cells induced by 2-methoxyethanol. Biotium and MTT assays were used to analyze neurons, which were isolated from the cerebral cortex of fetal mice at gestation day 19 (GD-19). A standard solution of 2-methoxyethanol was dosed at 10 μL. The cultured cells were randomly divided into the following groups: (1) 2-ME group+resveratrol standard, (2) 2-ME group+resveratrol isolated from tempeh, (3) 2-ME group+resveratrol isolated from soybean seed coats, and (4) the control group, without the addition of either 2-ME or resveratrol. Exposure of the primary cortical neuron cells to beta-amyloid monoclonal antibody pre-incubated for 24 h with 10 µL of 4.2 µg/mL resveratrol and 7.5 mmol/l 2-methoxy-ethanol additions. Here, we report that the addition of 2-ME and resveratrol (standard and isolated from tempeh) of cell culture at concentrations of 1.4, 2.8 and 4.2 µg/mL showed that the majority of neurons grew well. In contrast, after exposure to 2-ME and Beta-amyloid, showed that glial activated. These findings demonstrate a role for resveratrol in neuroprotective-neurorescuing action. © 2022, Instituto Internacional de Ecologia. All rights reserved.

2-methoxyethanol; Neuroprotective; Neurorescuing; Neurotoxic; Resveratrol


Brazilian Journal of Biology

Publisher: Instituto Internacional de Ecologia

Volume 82, Issue , Art No e235781, Page – , Page Count

Journal Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103600841&doi=10.1590%2f1519-6984.235781&partnerID=40&md5=f9c209be3a962349c4dffbe941a9af26

doi: 10.1590/1519-6984.235781

Issn: 15196984

Type: All Open Access, Gold


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