Molecular analysis of ompA gene pasteurella multocida Indonesia local isolates

Dewi A., Estoepangestie A., Suwarno S., Handijatno D., Ernawati R., Tyasningsih W.

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Universitas Airlangga, Surabaya, 60115, Indonesia


Abstract

The aim of this research was to analyze ompA molecular gene of Pasteurella multocida buffalo isolate and bovine isolate from Nusa Tenggara Timur, Indonesia and Katha strain isolate from hemorrhagic septicemia vaccine. Determinant of P. multocida local isolates ompA gene amplification sequencing PCR then conducted to see the sequence of nucleotide sequences of ompA gene. The results of PCR amplification showed an amplicon of 559 bp of all isolates. The homology analysis result of the isolates ranged from 93 – 100% with 13 P. multocida isolates from GenBank, and phylogenetic tree analysis shows that buffalo isolate was closely related to Katha strain, Iran, India and China isolate. Whereas bovine isolate far enough with buffalo and Katha strain isolate. Nucleotide sequences were compared to amino acids then by the method of Kolaskar and Tongaonkar antigenicity predicted antigens in P. multocida. B cell epitope predictions from local isolates and Katha strain were found in five peptides QVSPVFAG, IPELALRVEYQ, GQSVYVPEVVSKT, LKSASVAVAG, and ANYLVAKG. © 2021, College of Veterinary Medicine, University of Mosul.

Local isolate; Nucleotide; OmpA gene; Pasteurella multocida


Journal

Iraqi Journal of Veterinary Sciences

Publisher: University of Mosul – College of Veterinary Medicine

Volume 35, Issue 2, Art No , Page 211 – 216, Page Count


Journal Link: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104134772&doi=10.33899%2fijvs.2019.125934.1191&partnerID=40&md5=827c905b18d14e81f840b9ecdb4e7fcf

doi: 10.33899/ijvs.2019.125934.1191

Issn: 16073894

Type: All Open Access, Gold


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