Immunostimulatory activity test of extract and isolation of the secondary metabolites of kabau (Archidendron bubalinum) fruit peel

Okta F.N., Sukrasno S., Anggadiredja K.

Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia


Kabau fruit peel has higher antioxidant activity. Antioxidants can changes immunological and oxidative stress parameters and enhance the immune system. This study aimed to determine the immunostimulatory activity of 96 % and 50 % ethanol extracts of kabau fruit peel and to isolate the major secondary metabolites. The immunostimulatory activity was tested using the carbon clearance method. Eighteen male Swiss Webster mice were divided into groups, given each ethanol extract at a respective dose of 100 and 200 mg/kg BW, 0.5 % Na-CMC as the negative control, and 19.5 mg/kg BW Stimuno as the positive control. Each treatment was given for seven days. The active extract was further fractionated and isolated using chromatography, followed by identification and characterization of the isolate using TLC-Scanner and NMR. The 96 % ethanol extract had immunostimulatory activity, with a phagocytic index of 1.17. Isolation of the ethanol extract revealed Quercitrin, a flavonoid, with Rf value of 0.55. Results of this study indicate that kabau fruit peel extract has immunostimulatory activity with flavonoid as the active ingredient. © 2021 by the authors. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (

Carbon clearance; Flavonoids; Fruit peel; Immunomodulatory; Kabau (Archidendron bubalinum)


Biointerface Research in Applied Chemistry

Publisher: AMG Transcend Association

Volume 12, Issue 2, Art No , Page 2691 – 2700, Page Count

Journal Link:

doi: 10.33263/BRIAC122.26912700

Issn: 20695837



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